First characterized 30 years ago, aromatic L-amino acid decarboxylase (AADC) deficiency is a rare, genetic disorder that affects the central nervous systems of young patients.[1,2] AADC deficiency is caused by defect in the dopa decarboxylase (DDC) gene, which leads to a reduction in the critical neurotransmitters dopamine, norepinephrine, epinephrine, and melatonin in the brain.
Aromatic L-Amino Acid Decarboxylase (AADC) Deficiency Overview
Ashutosh Kumar, MD, Division of Neurology, Penn State Children’s Hospital
PATHOPHYSIOLOGY AND EPIDEMIOLOGY
The DDC gene creates an enzyme that is required for the production of the neurotransmitters dopamine, epinephrine, norepinephrine, serotonin, and melatonin. Therefore, when the enzyme is not present, the levels of all of these neurotransmitters are far lower than normal, interfering in several ways with intercellular communication. The abnormal neurotransmitter levels inhibit motor and muscle development, as described above.[2,5]
This genetic disorder is passed down through autosomal recessive heredity; meaning that only children receiving a mutated DDC gene from both parents can express symptoms. Those receiving only one copy of a mutation will have normal DDC gene function but are carriers of the recessive, defective gene. Yet, over 80 variants of the DDC gene have been recorded, which probably explains the spectrum of symptoms seen in patients.
AADC deficiency is an extremely rare disorder: From 1990 to 2020, case series of 120 to 130 patients have been published.[5,7] Interestingly, a new study asserts that AADC deficiency may be a bit more common: Researchers announced findings in 2020 that AADC deficiency may be associated with some neurologic disorders in children.[3,8] They believe that perhaps 1 in 900 patients (up to 20 years old) with undiagnosed neurotransmitter disorders may be attributable to AADC deficiency.
AADC deficiency has been documented all over the world, but the highest prevalence is seen in the Asian population due to the founder effect. Based on the case series, the average age when symptoms first appear is 2.7 months, but mean age at time of first diagnosis is age 3.5 years.
SIGNS AND SYMPTOMS
The first sign of the disease is usually hypotonia (e.g., the neck muscles are too weak to hold the baby’s head up). Other neurological problems, including developmental delay (e.g., walking and talking), movement disorders (e.g., involuntary movements), and autonomic issues (e.g., excessive sweating, chronic nasal congestion, hypotension, uncontrolled heart rate, and ptosis), are common; patients may have one or more of these signs. Other reported signs in AADC deficiency are frequent sleep disturbances, which are caused by the melatonin abnormalities, general lack of energy, and poor appetite. Chronic diarrhea was noted in a case series of 10 French children with the disorder.
One movement disturbance that can be extremely concerning to caregivers of patients with AADC deficiency is a syndrome called oculogyric crisis, prolonged involuntary upward deviation of the eyes. Symptoms tend to worsen with fatigue and improve after the patient sleeps.
Once suspected, a diagnosis is challenging: analysis of metabolites in cerebrospinal fluid, assessment of plasma AADC activity, and/or DNA sequence analysis is required. Blood testing and cerebrospinal fluid analysis will show very low levels of specific chemicals (e.g., 5-hydroxyindoleacetic acid) related to the production of the neurotransmitters dopamine and serotonin. Blood tests can measure the activity of the enzyme that is encoded by the DDC gene. Genetic testing for DDC mutations will confirm the diagnosis.
MANAGEMENT AND CURRENT TREATMENT
Unfortunately, AADC deficiency is not yet curable and there is no effective treatment. Best practice involves raising the patient’s levels of the deficient neurotransmitters, in the hope that it will mitigate the child’s signs and symptoms. In some patients, combinations of medications can help alleviate individual symptoms, but no one strategy works well for all patients. To an extent, the success of these efforts depend on the body’s ability to produce naturally functioning AADC enzyme.
According to current consensus-based treatment guidelines, the medication classes (alone or in combination) are listed in the Table below. Each medication is associated with significant side effects and/or drug interactions, and customization of treatment is important. Also, all of these treatments are off-label use for AADC deficiency and proper clinical trials are still need to determine if these threapies are effective.
According to the Pediatric Neurotransmitter Disease Association, there is evidence of effectiveness of physical, occupational, and speech therapy for patients with AADC deficiency.
Table. Medications that may be effective in treating a patient with AADC deficiency
|Medication Class||Rationale||Examples of Preferred Drugs|
|Dopamine agonists||Directly activate postsynaptic dopamine receptors||Pramipexole, ropinirole, rotigotine|
|MAO inhibitors*||Prevent the breakdown of dopamine and serotonin||Tranylcypromine, selegiline, phenelzine|
|Vitamin B6||May increase activity of naturally available AADC enzyme||Pyridoxine|
|Anticholinergic agents*||Used to treat autonomic symptoms, dystonia, and
|Melatonin||May help improve sleep patterns||Melatonin|
|Botulinum toxin||Alternative for treating dystonia||—|
|Benzodiazepines||May improve dystonia||Clobazam, diazepam|
|Clonidine||Used to treat irritability and sleep disturbances||—|
|Nasal decongestants||Alleviates nasal congestion||Oxymetazoline, xylometazoline drops|
|L-Dopa†||Forms a substrate to encourage production of dopamine||L-dopa (without carbidopa)|
*Often used in combination with other medication classes.
†Used in special cases—with known L-dopa binding site variant, or as a third-line agent.
COMPLICATIONS OF AADC DEFICIENCY
There is little information based on long-term follow-up of patients with AADC deficiency. One review reports that the autonomic symptoms of the disorder can be life threatening, and problems like joint contracture could become apparent because of long-term musculoskeletal issues.
The consensus guideline recommends vigilance against cardiac complications, such as arrhythmia, based on the autonomic symptoms of AADC deficiency. In addition, impaired feeding and swallowing could raise the risk of infections (including pneumonia) in these patients.
NEW TREATMENT DIRECTIONS
As an autosomal genetic disorder, gene therapy would seem to be a reasonable approach. In fact, early attempts at gene therapy have been conducted with Asian patients, utilizing adenovirus vectors to introduce normal DDC genes into the body. This treatment, called AGIL-AADC or PTC-AADC, resulted in improved body weight gain, less dyskinesia, and lower frequency of oculogyric crises 12 months after injection, based on two open-label studies. None of the 18 patients (21 mo to 8.5 yr) had full head control or could sit unassisted or stand at baseline. All patients achieved clinically meaningful, sustained improvements (up to 5 yr for some patients) in motor, cognitive, and language milestones. The researchers reported that no new safety signals were demonstrated.[12,13]
According to the drug maker, PTC Therapeutics, an application for approval for AGIL-AADC could be filed with the Food and Drug Administration in 2020.
CLINICAL TRIAL INFORMATION
Please visit Clinicaltrials.gov to research clinical trials currently underway. This link addresses trials in AADC deficiency that may be recruiting or in progress.
National Organization for Rare Disorders (NORD)
Provides a unified voice for the 30 million people who wake up every day to fight the battle with a rare disease, including parents and caregivers.
- Hyland K, Clayton PT. Aromatic amino acid decarboxylase deficiency in twins. J Inherit Metab Dis.1990;13:301-304.
- Aromatic L-amino acid decarboxylase deficiency. National Institutes of Health Genetic and Rare Disease Information Center. July 14, 2017 https://rarediseases.info.nih.gov/diseases/770/aromatic-l-amino-acid-decarboxylase-deficiency. Accessed March 24, 2020.
- Hyland K, Reott M. Prevalence of aromatic L-amino acid decarboxylase deficiency in at-risk populations. Pediatr Neurol. Dec 26, 2019 [Epub ahead of print]. doi: 10.1016/j.pediatrneurol.2019.11.022.
- Kojima K, Anzai R, Ohba C, et al. A female case of aromatic l-amino acid decarboxylase de¡ciency responsive to MAO-B inhibition. Brain Dev. 2016;38:959-963.
- Wassenberg T, Molero-L M, Jeltsch K, et al. Consensus guideline for the diagnosis and treatment of aromatic L-amino acid decarboxylase (AADC) deficiency. Orphanet J Rare Dis. 2017;12:12. DOI: 10.1186/s13023-016-0522-z.
- Himmelreich N, Montioli R, Bertoldi M, et al. Aromatic amino acid decarboxylase deficiency: Molecular and metabolic basis and therapeutic outlook. Mol Genet Metab. 2019;127:12-22.
- Dai W, Lu D, Gu X, Yu Y. Aromatic L-amino acid decarboxylase deficiency in 17 Mainland China patients: Clinical phenotype, molecular spectrum, and therapy overview. Mol Genet Genomic Med. 2020;8:e1143. doi: 10.1002/mgg3.1143.
- Pataia V. AADC Deficiency may be ‘common’ in neurological problems of unknown origin. AADC News. March 17, 2020. https://aadcnews.com/2020/03/17/aadc-deficiency-more-common-to-neurological-problems-unknown-origin-that-thought/. Accessed March 25, 2020.
- Lee HF, Tsai CR, Chi CS, Chang TM, Lee HJ. Aromatic L-amino acid decarboxylase deficiency in Taiwan. Eur J Paediatr Neurol. 2009;13(2):135–40.
- Spitz MA, Nguyen MA, Roche S, et al. Chronic diarrhea in L-amino acid decarboxylase (AADC) deficiency: A prominent clinical finding among a series of ten French patients. JIMD Rep. 2017;31:85-93. doi: 10.1007/8904_2016_550.
- What is aromatic L-amino acid decarboxylase deficiency? Pediatric Neurotransmitter Disease Association. http://www.pndassoc.org/diseases/aadc.html. Accessed March 27, 2020.
- Hwu W-L, Chien Y-H, Lee N-C, et al. Safety and improved efficacy outcomes in children with AADC deficiency treated with AGIL-AADC gene therapy: Results from three clinical trials (abstract). Presented at the 48thAnnual Meeting of the Child Neurology Society, Oct 23-26, 2019, Charlotte, NC, USA. Poster P231. https://www.childneurologysociety.org/docs/default-source/2019-CNS/2019-cns-program.pdf?sfvrsn=2. Accessed March 27, 2020.
- Chien Y-H, Lee N-C, Tseng S-H, et al. AGIL-AADC gene therapy results in sustained improvements in motor and developmental milestones through 5 years in children with AADC deficiency (abstract). Presented at the 48thAnnual Meeting of the Child Neurology Society, Oct 23-26, 2019, Charlotte, NC, USA. Poster P207. https://www.childneurologysociety.org/docs/default-source/2019-CNS/2019-cns-program.pdf?sfvrsn=2Accessed March 27, 2020.
- PTC Therapeutics announces results from long-term AADC deficiency gene therapy treatment demonstrating sustained improvements (press release). PTC Therapeutics. October 24, 2020. http://ir.ptcbio.com/news-releases/news-release-details/ptc-therapeutics-announces-results-long-term-aadc-deficiency. Accessed March 27, 2020.
Diagnosing Aromatic L-Amino Acid Decarboxylase (AADC) Deficiency
Kelly Heger Discusses AADC Deficiency and Her Family’s Diagnostic Journey
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